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[ Care and Use ManUal ]

PIC Reagents 1

[ Care and Use ManUal ]

Contents

I. IntroduCtIon

II. PaIred-Ion ChromatograPhy

III. seleCtIng the reagent for your seParatIon

IV. usIng and storIng Waters PIC reagents

V. orderIng InformatIon

VI. Warranty/serVICe InformatIon

I. IntroduCtIon

Waters PIC

reagents allow simultaneous separation of acids, bases,

amphoteric, and neutral compounds on a reversed-phase column

by paired-ion chromatography (PIC). Separation of ionic species in

complex samples is easy with PIC reagents.

Use PIC A for separation of acids.

Use the PIC B series to separate bases.

Use Reagent D4 as a mobile phase modifier when separating basic

pharmaceutical compounds to improve peak shape and reduce reten-

tion times.

Low-UV PIC reagents are also available, for use in separations of

compounds that have chromophores below 240 nm.

Table 1. PIC Reagents

PIC reagents

II. PaIred-Ion ChromatograPhy

Paired-ion chromatography is a technique for performing ion-

exchange separations on a reversed-phase column. Hydrophobic

paired-ion reagents added to the mobile phase are adsorbed onto the

stationary phase. There they enhance the interaction of oppositely

charged sample components with the stationary phase. A paired-ion

reagent:

• Increasestheretentionofoppositelychargedsolutes

• Decreasestheretentionofequallychargedsolutes

• Doesnotaffecttheretentionofunchargedsolutes

For a detailed description of the retention mechanism, see B.A.

Bidlingmeyer, S.N. Deming, W.P. Price, B. Sachok and M. Petrusek,

“Retention Mechanism for Reversed-Phase Ion-Pair Liquid Chromatog-

raphy”, Journal of Chromatography 186 (1979), 419-434.

III. seleCtIng the reagent for your seParatIon

Use PIC A Reagent to increase the retention of acids. Use the PIC B

Reagent to increase the retention of bases. Which of the four B

reagents to use depends upon your sample. Retention increases with

increasing reagent chain length. It is usually best to start with PIC B7.

If more retention is needed, use PIC B8. If less is needed, use PIC B5

or B6.

You can also use the concentration of organic modifier in your mobile

phase to manipulate the retention of all compounds, including neutral

compounds.

Reagent Compound

PIC A Tetrabutylammonium phosphate

Low UV PIC A Tetrabutylammonium hydrogen sulfate

PIC B5 Pentane sulfonic acid

PIC B6 Hexane sulfonic acid

PIC B7 Heptane sulfonic acid

PIC B8 Octane sulfonic acid

Reagent D4 Dibutylammonium phosphate

1 2 3

Inhaltsverzeichnis

Seite 1 - PIC reagents

[ Care and Use ManUal ]PIC Reagents 1[ Care and Use ManUal ]ContentsI. IntroduCtIonII. PaIred-Ion ChromatograPhyIII. seleCtIng the reagent for y

Seite 2

[ Care and Use ManUal ]PIC Reagents 2[ Care and Use ManUal ]Column: µBondapak C18, 3.9 x 300 mm steel column Part Number: WAT027324Mobile Phas

Seite 3

[ Care and Use ManUal ]PIC Reagents 3Sales Offices:Austria and European Export (Central South Eastern Europe, CIS and Middle East) 431 877 18 07Austra

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Waters PIC Reagents Bedienungsanleitung

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