Waters MassPREP Phosphopeptide Standards Bedienungsanleitung PDF herunterladen

[ Care and Use ManUal ]

Phosphopeptide Standards 1

Contents

I. IntroduCtIon

II. synthetIC enolase PhosPhoPePtIdes

III. reCommended usage

IV. samPle PreParatIon

V. examPle sPeCtra

VI. storage and stabIlIty

VII. related aCCessorIes

massPreP PhosPhoPePtIde standards

I. IntroduCtIon

Phosphorylated proteins and peptides are difficult to detect and

characterize by mass spectrometry due to their low abundance and

low ionization efficiency. A set of standard samples can aid in devel-

oping methods for optimized phosphopeptide detection. As a tool

for screening sample preparation and sample enrichment methods

for phosphopeptides, Waters offers MassPREP™ Phosphopeptide

Standards containing synthetic phosphopeptides based on modified

peptides from tryptic yeast enolase digest (SwissProt P00924).

Commonly used phosphorylated samples are ß-casein digests or

standards which contain only two phosphopeptides, both modified

atserine. The four modified enolase peptides in the mixture are either

singly phosphorylated at serine, threonine, or tyrosine or doubly

phosphorylated at serine.

II. synthetIC enolase PhosPhoPePtIdes

Peptide Sequence Isotopic mass,

[M+2H]2+

[M+H]+

T18 1P NVPLpY K 813.3912 407.1995

T19 1P HLADL pSK 863.4028 432.2053

T43 1P VNQIG pTLSES IK 1368.6776 684.8428

T43 2P VNQIG TLpSEpS IK 1448.6439 724.8259

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[ Care and Use ManUal ] 1

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[ Care and Use ManUal ]Phosphopeptide Standards 1ContentsI. IntroduCtIonII. synthetIC enolase PhosPhoPePtIdesIII. reCommended usageIV. samPle PreParat

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[ Care and Use ManUal ]Phosphopeptide Standards 2III. reCommended usageThere are three sample kits available (#1-3 below). Each kit contains 1 nmol ea

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[ Care and Use ManUal ]Phosphopeptide Standards 3V. EXAMPLE SPECTRAThe following MALDI-MS spectra (positive reflectron) of Phosphopep-tide Standard –

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Waters MassPREP Phosphopeptide Standards Bedienungsanleitung

Stöbern Sie online oder laden Sie Bedienungsanleitung nach Wasserausrüstung Waters MassPREP Phosphopeptide Standards herunter. Waters MassPREP Phosphopeptide Standards User Manual Benutzerhandbuch

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