Waters PAH Columns Bedienungsanleitung PDF herunterladen

[ Care and Use ManUal ]

Name of Document 1

Contents

I. IntroduCtIon

II. ConneCt Ing the Column to the hP lC Inst rument

a. Column Connectors and System Tubing Considerations

b. SLIPFREE Connectors

c. Minimization of Band Spreading

d. Measuring System Bandspread Volume

e. Measuring Gradient Delay Volume

f. Use of Narrow-Bore Columns (3.0 mm i.d.)

g. Impact of bandspreading on Column performance

(2.1 mm i.d. column)

h. System Modification Guidelines

III. Column equIlIbratIon

IV. Column usage

V. Column CleanIng, regeneratIng and storage

a. Cleaning and Regeneration

b. Storage

VI. t roubleshootIng

Waters Pah Column

I. IntroduCtIon

We are sure you will find that Waters built-in quality helps solve many of

your challenging separation problems. We strive to provide products with the

highest degree of lot-to-lot and column-to-column reproducibility to mini-

mize variations in your chromatographic results. Waters PAH columns are

manufactured and packed under highly controlled conditions. Each must pass

a series of stringent tests before being accepted for shipment. Included with

each column is the final Certificate of Analysis.

Polynuclear Aromatic Hydrocarbons (PAHs) are among the most frequently

monitored environmental contaminants. Standard and official methods for the

analysis of PAHs are found in compendia for air, drinking water, wastewater,

solid waste, and food analysis1.

Many of these methods specify HPLC, usually with UV and fluorescence detec-

tion, as the recommended analytical procedure.

Waters PAH columns are optimized for the HPLC analysis of PAHs. Figure 1

shows a chromatogram of 16 PAH compounds, listed as target pollutants by

the U.S. EPA. The Waters PAH columns achieve baseline resolution and excel-

lent peak symmetry for all 16 target analytes in less than 25 minutes, while

employing a simple water; acetonitrile binary gradient. The resolving power

of the PAH Columns provides superior peak identification and quantitation

for PAHs.

Florida Administrative Code 17.700 includes 2 additional compounds

(1-methyl naphthalene and 2-methyl naphthalene ) in addition to the 16 com-

pound EPA 610 mix that we currently use to show the proficiency of Waters

instrumentation to analyze PAH compounds. The new Waters PAH columns

resolve these two compounds along with the other 16, (see Figure 2).

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Inhaltsverzeichnis

Seite 1 - Waters Pah Column

[ Care and Use ManUal ]Name of Document 1ContentsI. IntroduCtIonII. ConneCt Ing the Column to the hP lC Inst rumenta. Column Connectors and Syste

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[ Care and Use ManUal ]Name of Document 2Column: Waters PAH Column 5 μm 4.6 x 250 mm @ 27 °C Peaks:System: Waters Alliance Syste

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[ Care and Use ManUal ]Name of Document 33. If a leak occurs between the stainless steel compression screw fitting and the column endfitting, a new

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[ Care and Use ManUal ]Name of Document 4b. SLIPFREE ConnectorsGuarantees a void-free connection because it pushes the tubing into the endfitting; Thi

Seite 5

[ Care and Use ManUal ]Name of Document 5Figure 5: Determination of Dwell Volumef. Use of Narrow-Bore Columns – (3.0 mm i.d.)This section describes ho

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[ Care and Use ManUal ]Name of Document 6Note: If mobile phase additives are present in low concentrations (such as ion-pairing reagents, at 5 to 10 m

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[ Care and Use ManUal ]Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.comName of Document

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Waters PAH Columns Bedienungsanleitung

Stöbern Sie online oder laden Sie Bedienungsanleitung nach Wasserausrüstung Waters PAH Columns herunter. Waters PAH Columns User Manual Benutzerhandbuch

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