Waters BioSuite Peptide Analysis Columns Bedienungsanleitung PDF herunterladen

[ Care and Use ManUal ]

BioSuite Peptide Analysis Columns 1

Contents

I. IntroduCtIon

a. Column Performance Validation

b. Waters BioSuite C

3 μm PA-A and BioSuite C

3.5 μm

PA-B Columns

c. BioSuite C

3 μm, PA-A

d. BioSuite C

3 μm, PA-B

e. Operating Conditions

II. sample preparatIon

III. mobIle phase ConsIderatIons

IV. Flow dIreCtIon

V. ConneCtIng your Column to the hplC system—

Column ConneCtIon

VI. Column ConneCtors and system tubIng

ConsIderatIons

a. Column Performance Validation

VII. measurIng system bandspread Volume

VIII. measurIng gradIent delay Volume

IX. use oF bIosuIte “narrow-bore” peptIde analysIs

Columns (I.e., 2.1 mm I.d.)

X. system modIFICatIon reCommendatIons For bIosuIte

2.1 mm I.d. peptIde analysIs Columns

XI. addItIonal ConsIderatIons when usIng bIosuIte C

peptIde analysIs Columns

I. IntroduCtIon

Waters BioSuite

™

high performance chemistries are dedicated to the isola-

tion, analysis, and characterization of proteins and peptides. This Care

and Use document details the use of Waters BioSuite Peptide Analysis

Columns designed for peptide mapping applications. For the latest list

of BioSuite ion-exchange, size exclusion, hydrophobic interaction, and

reversed-phase columns, go to the “Life Sciences Chemistry Section” at

www.waters.com/lifesciences.

PLEASE READ CAREFULLY BEFORE USING THIS COLUMN

Thank you for choosing a Waters BioSuite Peptide Analysis Column. Every

batch of packing material is specifically tested using a tryptic digest of

cytochrome c. Only those batches that pass stringent tolerance values are

selected for use in BioSuite C

3 μm PA-A or C

3.5 μm PA-B Columns.

a. Column Performance Validation

Each BioSuite Peptide Analysis Column comes with a Certificate of Batch

Analysis and a Performance Test Chromatogram. The Certificates of Batch

Analysis are specific to each batch of packing material contained in the

BioSuite Peptide Analysis Column and includes the gel lot batch number,

analysis of unbonded particles, analysis of bonded particles, and chromato-

graphic results and conditions. The Performance Test Chromatogram is spe-

cific to each individual column and contains information such as gel lot batch

number, column serial number, USP plate count, USP tailing factor, capacity

factor and chromatographic results and conditions. These data should be

stored for future reference.

b. Waters BioSuite C

3 μm PA-A and BioSuite C

3.5 μm

PA-B Columns

BioSuite PA Series consists of two of Waters premier reversed-phase column

chemistries specifically voptimized for peptide mapping from simple to com-

plicated digests.

c. BioSuite C

3 μm, PA-A

BioSuite C

3 μm PA-A is a 100Å, diffunctional bonded, low ligand

density, silica-based column.

• Specificallydesignedforexcellentretentionofpolarpeptides

• IdealchoiceforLC/MSapplicationsusingformicacid(FA)that 

minimizes ion-suppression

• ExcellentperformancefortraditionalHPLC-separationsusinglow

TFAconcentrations(e.g.,0.025%TFA)

bIosuIte peptIde analysIs Columns

1 2 3 4 5 6 7 8

[ Care and Use ManUal ] 1

Inhaltsverzeichnis

Seite 1 - [ Care and Use ManUal ]

[ Care and Use ManUal ]BioSuite Peptide Analysis Columns 1ContentsI. IntroduCtIon a. Column Performance Validation b. Waters BioSuite C18 3

Seite 2

[ Care and Use ManUal ]BioSuite Peptide Analysis Columns 2d. BioSuite C18 3 μm, PA-BBioSuite C18 3 μm, PA-B is a 300Å, high ligand density, monofuncti

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[ Care and Use ManUal ]BioSuite Peptide Analysis Columns 32. ThedatainTable1wasgeneratedusingaBioSuiteC18, 3 μm PA-A 4.6mmx250mmanda

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[ Care and Use ManUal ]BioSuite Peptide Analysis Columns 4IV. Flow dIreCtIonThe recommended flow direction through the column is indicated by the arro

Seite 5

[ Care and Use ManUal ]BioSuite Peptide Analysis Columns 5Inapropertubing/columnconnection(Figure2),thetubingtouchesthebottom of the

Seite 6

[ Care and Use ManUal ]BioSuite Peptide Analysis Columns 6a. Minimizing Peak Band BroadeningFigure 6 shows the influence of tubing internal diameter o

Seite 7

[ Care and Use ManUal ]BioSuite Peptide Analysis Columns 7Note: Flow splitters after the column will introduce additional bandspread-ing. System optim

Seite 8

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Inhaltsverzeichnis

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