Waters XBridge Direct Connect HP 2.1 x 30 mm Column for A Bedienungsanleitung PDF herunterladen

[ Care and Use ManUal ]

XBridge Direct Connect HP 2.1 x 30 mm Column

The XBridge

™

Direct Connect HP 2.1 x 30 mm column makes it possible

to analyze specific analytes in a sample matrix when combined with

Waters ACQUITY UPLC

columns and online SPE technology.

CONTENTS

I. INTRODUCTION

II. SAMPLE PREPARATION

III. EQUIPMENT

IV. TYPICAL CHROMATOGRAPHIC CONDITIONS

1. INTRODUCTION

The XBridge packing materials were designed to provide excellent

peak shape, high efficiency, and excellent stability for acidic and basic

mobile phases. The recommended operating pH limits for XBridge Direct

Connect HP columns are pH 1-12. For more information on XBridge

HPLC analytical columns, please refer to the XBridge Columns Care and

Use Manual (P/N 720001366EN). The XBridge Direct Connect column

can be screwed directly into the switching valve or connected to fit-

tings like a conventional HPLC column.

XBRIDGE DIRECT CONNECT HP 2.1 x 30 mm COLUMN FOR AUTOMATED SAMPLE

PREPARATION WITH WATERS UPLC WITH ONLINE SPE TECHNOLOGY

II. SAMPLE PREPARATION

Aqueous Samples: Water, Beverages

Adjust pH to maximize analyte retention on the SPE sorbent. Buffer

salts and dispersive agents may be used to increase partitioning onto

the SPE sorbent. Pretreatment to remove suspended matter prior to

SPE treatment may include filtration or centrifugation.

Non-Aqueous Liquid

When appropriate the sample may be diluted with aqueous buffers

and organic co-solvents for reversed-phased SPE. If sufficient dilution

has occurred, the sample may be treated in a manner similar to an

aqueous sample.

III. EQUIPMENT

Shown in Figure 1 is a schematic of Waters UPLC

system with online

SPE technology, which consists of a 2777 high-pressure sample

manager, an ACQUITY UPLC

quaternary solvent manager (QSM), an

ACQUITY UPLC binary solvent manager (BSM), two extraction columns,

an analytical column, and detection system (mass spectrometry, PDA

or fluorescence). The extraction columns are connected to a switching

valve so they can be operated in parallel.

2777c

Detector

ACQUITY

QSM

ACQUITY

BSM

SPE

Injection

Extraction

Column A

ACQUITY BEH Column

Extraction

Column B

Waste

2.0 mL/min

0.4 mL/min

Valve A: Injection valve for online SPE LC-MS/MS

Valve B: switching valve for extraction columns

Valve C: injection valve for stand-alone LC-MS/MS

B C

Figure 1: Schematic of Waters UPLC System with Online SPE Technology.

1 2 3

[ Care and Use ManUal ] 1

Inhaltsverzeichnis

Seite 1 - [ Care and Use ManUal ]

[ Care and Use ManUal ]XBridge Direct Connect HP 2.1 x 30 mm Column 1The XBridge™ Direct Connect HP 2.1 x 30 mm column makes it possible to analyze sp

Seite 2

[ Care and Use ManUal ]XBridge Direct Connect HP 2.1 x 30 mm Column 2IV. TYPICAL CHROMATOGRAPHIC CONDITIONS The analytical process begins with loadin

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[ Care and Use ManUal ]XBridge Direct Connect HP 2.1 x 30 mm Column 3Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F:

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Waters XBridge Direct Connect HP 2.1 x 30 mm Column for A Bedienungsanleitung

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