Waters High Strength Silica Columns Bedienungsanleitung PDF herunterladen

[ Care and Use ManUal ]

HSS HPLC Columns

Contents

I. IntRoDUCtIon

II. ConeCtInG tHe CoLUMn to tHe HPLC sYsteM

III. WateRs sMaLL PaRtICLe sIze (3.5 �M) CoLUMns

– Fast CHRoMatoGRaPHY

IV. CoLUMn eqUILIbRatIon

V. CoLUMn InstaLLatIon PRoCeDURe

VI. CoLUMn PeRFoRManCe VaLIDatIon

VII. InItIaL CoLUMn eFICIenCY DeteRMInatIon

VIII. CoLUMn UsaGe

IX. CoLUMn CLeanInG, ReGeneRatInG anD stoRaGe

X. tRoUbLesHootInG

I. IntRoDUCtIon

Thank you for choosing a High Strength Silica (HSS) HPLC column.

The manufacture of HSS HPLC columns begins with ultrapure

reagents to control the chemical composition and purity of the

final product. HSS HPLC columns are manufactured in a cGMP,

ISO 9001:2000 certified plant with each step being conducted

within narrow tolerances. Every column is individually tested and

Certificates of Batch Analysis and a Performance Chromatogram are

provided with each column.

HSS HPLC columns are based on the same particle technology and

chemistries as ACQUITY UPLC HSS columns, thus enabling seamless

transferability between HPLC and UPLC separations.

Waters recommends the use of Sentry

™

guard columns to maximize

column lifetime and protect the column from contaminants.

HIGH stRenGtH sILICa HPLC CoLUMns

1 2 3 4 5 6 7 8

[ Care and Use ManUal ] 1

Inhaltsverzeichnis

Seite 1 - [ Care and Use ManUal ]

[ Care and Use ManUal ]HSS HPLC Columns1ContentsI. IntRoDUCtIonII. ConeCtInG tHe CoLUMn to tHe HPLC sYsteMIII. WateRs sMaLL PaRtICLe sIze (3.5 �M) CoL

Seite 2

[ Care and Use ManUal ]HSS HPLC Columns2II. CONNECTING THE COLUMN TO THE HPLC SYSTEMa. Column ConnectionHandle the column with care. Do not drop or hi

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[ Care and Use ManUal ]HSS HPLC Columns3Figure 4: Waters Ferrule in a Parker Style EndfittingThere are two ways to fix the problem:1. Tighten the sc

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[ Care and Use ManUal ]HSS HPLC Columns4e. Measuring Gradient Delay Volume1. Replace the column with a zero dead volume union.2. Prepare eluent A (p

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[ Care and Use ManUal ]HSS HPLC Columns5V. CoLUMn InstaLLatIon PRoCeDURe Note: The flow rates given in the procedure below are for a typical 4.6 mm i.

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[ Care and Use ManUal ]HSS HPLC Columns6b. Sample Preparation 1. Sample impurities often contribute to column contamination. Use Waters Oasis® or Sep

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[ Care and Use ManUal ]HSS HPLC Columns7XIII. CoLUMn CLeanInG, ReGeneRatInG anD stoRaGe a. Cleaning and RegenerationA sudden increase in pressure or s

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[ Care and Use ManUal ]Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.comHSS HPLC Columns8

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Waters High Strength Silica Columns Bedienungsanleitung

Stöbern Sie online oder laden Sie Bedienungsanleitung nach Wasserausrüstung Waters High Strength Silica Columns herunter. Waters High Strength Silica Columns User Manual Benutzerhandbuch

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