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[ CARE AND USE MANUAL ]

MassPREP Protein Standard Mix 1

I. INTRODUCTION

The MassPREP

™

Protein Standard Mix is a mixture of a variety of carefully

chosen proteins encompassing a wide range of properties. This mixture

contains proteins that vary in isoelectric points, molecular weights and

hydrophobicities. These characteristics provide the user with an attractive

intact protein mixture that can be used for a variety of applications.

Table 1 describes the composition of this mixture.

The standard was developed to provide a means to test Waters UPLC

and HPLC Protein Separation Columns such as the ACQUITY UPLC

BEH C

1.7 μm and XBridge

™

BEH300, C

3.5 µm column offerings.

In our quest to offer useful analytical tools, we now offer the same

protein standard mix used at Waters to QC batch test of some of

our Protein Separation Technology Column offerings. The standard is

prepared under strict quality control criteria confirmed on the specific

certificate of analysis that is available upon request for each batch

of prepared MassPREP Protein standard. In addition to evaluation of

HPLC/UPLC columns, this mixture may also be used for a variety of

other applications, including evaluation of mass spectrometry and

chromatography instrumentation performance.

Table 1. Composition of MassPREP Protein Standard Mix

Protein Sample

Molecular

Weight, MW

SwissProt

Sequence

Information

Part

Number

(Sigma)

Ribonuclease A, bovine pancreas 13.700 K P61823* R5500

Cytochrome c, horse heart, 96% 12.384 K P00004* C7752

Albumin, bovine serum, 96-99% 66.430 K P02769* A8022

Myoglobin, horse heart >90%

16.950 K plus

the heme group

616 K

Tot al:17.600 K

P68082 M1882

Enolase, from baker’s yeast

(S. cerevisiae)

46.671 K P00924 E6126

Phosphorylase b, rabbit muscle 97,20 0 K P00489 P6635

*Best match from database for more information on sequence please

reference the raw material vendor information (Sigma)

MASSPREP PROTEIN STANDARD MIX

CONTENTS

I. INTRODUCTION

II. STORAGE AND STABILITY

III. RECONSTITUTION OF MASSPREP PROTEIN STANDARD MIX

FOR HPLC/UPLC USE

1 2 3

Inhaltsverzeichnis

Seite 1 - MASSPREP PROTEIN STANDARD MIX

[ CARE AND USE MANUAL ]MassPREP Protein Standard Mix 1I. INTRODUCTIONThe MassPREP™ Protein Standard Mix is a mixture of a variety of carefully chosen

Seite 2

[ CARE AND USE MANUAL ]MassPREP Protein Standard Mix 2II. STORAGE AND STABILITYUpon receiving the sample, store immediately at -20 °C. The lyophilize

Seite 3

[ CARE AND USE MANUAL ]Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.comMassPREP Protein

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Waters MassPREP Protein Standard Mix Bedienungsanleitung

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